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x�b```f`��e`g`��� Ȁ �l@Q�U0��o�t�Gr��� For DNA isolation, a single bacterial colony of isolate DS1 was inoculated into 5ml of a rich medium (gl 1; glucose, 2.0; glutamic acid, 1.5; peptone, 1.5; K 2HPO 4, 0.5; MgSO 4 7H 2O, 0.5; yeast extract, 2.0; pH, 6). 0
[2] In addition to being a part of the Acetobacter family, Gluconacetobacter diazotrophicus belongs to the Proteobacteria phylum, the Alphaprotebacteria class, and the Gluconacetobacter genus while being a part of the Rhodosprillales order. 0000041289 00000 n
[2] This feature allows the bacteria to work on nitrogen in the air in order for the correct amount of nitrogen can be received by plants. Extensive online help - available wherever you are in CAB Direct. [2] The G-C content for Gluconacetobacter diazotrophicus could be calculated at 66.19%. 112 108
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The genome that was found to be closely sequenced with Gluconacetobacter diazotrophicus was the Pal5 genome. Fifty-five G. diazotrophicus strains used in this work (Table 1) were isolated and characterized by microbiological, biochemical, and genetic tests in different studies [ 8, 11, 15 ]. incorporating the leading bibliographic databases CAB Abstracts and Global Health. 0000006139 00000 n
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[2] This genome has one circular chromosome and two plasmids. [9] In regard to the ecology of this microorganisms, the numbers of G. diazotrophicus that colonize sugarcane decrease when the plant is grown under high nitrogen fertilization doses. 0000127870 00000 n
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You can now claim your publications on CAB Direct with your ORCID iD! [2][3] Again, the bacterium’s name was changed to Gluconacetobacter diazotrophicus when its taxonomic position was resolved using 16s ribosomal RNA analysis. 0000003330 00000 n
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Gluconacetobacter diazotrophicus is a very well-known and well-studied member of family Acetobacteraceae. There are over 13,605,000 records available in CAB Direct | Last updated on November 26, 2020. CAB Direct is the most thorough and extensive source of reference in the applied life sciences,
[1][2] Based on these descriptions of the cell, Gluconacetobacter diazotrophicus can be classified with the bacillus genus. 0000113789 00000 n
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A diazotrophic bacterium, Gluconacetobacter diazotrophicus, was isolated from sugar cane, where it was found to occupy internal tissues at high cell densities (10 6 to 10 7 cells g-1; Fig. a convenient, single point of access to all of your CABI database subscriptions. 0000127986 00000 n
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15.5) and could grow on high sucrose concentrations (10%) and fix N 2. 0000044289 00000 n
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Gluconacetobacter- like diazotrophs were encountered as dense populations inside the root and stem tissues of sugarcane cultivated in ancient agricultural fields of the Nile Delta. 0000124206 00000 n
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[2][3], Originally found in Alagoas, Brazil, Gluconacetobacter diazotrophicus is a bacterium that has several interesting features and aspects which are important to note. Counts of >10 5 cells g -1 were recorded in root and stem samples. trailer
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Gluconacetobacter diazotrophicus is a Gram-negative, acid tolerant, obligate aerobe and the cells are rod shaped with rounded ends (0.7–0.9 µm by 1–2 µm) having lateral or peritrichous flagella 0000127391 00000 n
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[3] The cells be viewed as darkish brown or orange colonies under a microscope. 0000004071 00000 n
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Strains of this species have been isolated from all over the world. Find out more about this exciting new development, Using our new visualization tools you can, Using our new highlighting and annotation tool you can, remove selected records that are not saved in My CABI, sign you out of your
Gluconacetobacter diazotrophicus is an associative endophyte nitrogen fixing bacteria in sugarcane, is entirely confined to the living plant tissue, does not survive in soil and therefore can only be isolated by tissue extraction. Like most websites we use cookies. [10] Gluconacetobacter diazotrophicus cells are shaped like rods, have ends that are circular or round, and have anywhere from one to three flagella that are lateral. 0000042037 00000 n
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[1][2] The bacterium is known for stimulating plant growth and being tolerant to acetic acid. Gluconacetobacter diazotrophicus is also known for nitrogen fixation. 0000015981 00000 n
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Bacterial cultures have been preserved in 20% glycerol at −80 °C prior to analysis. 0000005981 00000 n
With one to three lateral flagella, and known to be found on sugarcane, Gluconacetobacter diazotrophicus was discovered in Brazil by Vladimir A. Cavalcante and Johanna Dobereiner. Gluconacetobacter diazotrophicus is a bacterium with a rod-like shape, has circular ends, and can be classified as a Gram-negative bacterium. 0000126629 00000 n