The percentage of reads on both ends of the ribosome reference sequence of all samples was less than 0.01% (Supplementary Table S2), and the total alignment rate with the reference genome of C. elegans was greater than 98.6% (Supplementary Table S3). Enter multiple addresses on separate lines or separate them with commas. 0000096300 00000 n
The expression of these two genes in nematodes was significantly reduced in the initial stage of infection in nematodes with P25. (Supplementary Materials). It can be seen that these DEGs were downregulated when nematodes were infected by K88, and they were upregulated in PR. Pediococcus acidilactici and Pediococcus pentosaceus are the most common species associated with opportunistic infections affecting compromised hosts. Golledge CL, Stingemore N, Aravena M, Joske D: Septicemia caused by vancomycin-resistantPediococcus acidilactici. Subsequent biochemical analysis showed all isolates to be negative for the production of pyrrolidonyl arylamidase (PYR test), positive for the production of leucine aminopeptidase and for the ability to grow in 6.5% NaCl, and resistant to vancomycin for a presumptive identification of the blood culture and catheter tip isolates as a Pediococcus species. The aqueous phase was collected, gently mixed with 600 μL isopropanol, and allowed to stand at 4°C for 30 min, then centrifuged at 12,000 rpm at 4°C for 15 min. Sign up here as a reviewer to help fast-track new submissions. 0000074763 00000 n
One patient with a concomitant Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa bacteremia was maintained on vancomycin with the addition of imipenem and fosfomycin. Phone: (202) 737-3600, Copyright © 2020 American Society for Microbiology | Privacy Policy | Website feedback, Print ISSN: 0095-1137; Online ISSN: 1098-660X, Sign In to Email Alerts with your Email Address. However, a significant difference () was observed between nematodes fed of K88 (attacked group, AT) and CK. The supernatant was carefully removed, and 1 mL 75% ethanol was added (in DEPC-treated water) to the gelatinous RNA pellets. qRT-PCR data were analyzed using the 2-ΔΔCt method to determine the relative abundances (fold changes) of the target genes [48]. Journal of Clinical Microbiology 1988, 26: 2064–2068. 0000075316 00000 n
The suspension was gradient diluted and plated onto EMB or MRS medium to determine the bacteria of the worm’s gut (). Pediococcus were present in both healthy and infected cows. M and D.D. 0000076268 00000 n
2 μL of each cDNA at 100 ng was included in 10 μL 2× iTaq Universal SYBR Green Supermix, 0.8 μL of each primer at 100 nM, and 6.4 μL ddH2O. Two weeks before farrowing, 30 sows and their future litters were allocated to the following treatments: 1) control group without antibiotic or probiotic treatment (CTRL), 2) control with antibiotic (tiamulin) added to weanling feed (ABT), or litters treated with 3) PA, 4) SCB, or 5) PA+SCB from 24 h after birth. Son, S. Oh, and Y. Kim, “Short communication: development of a direct in vivo screening model to identify potential probiotic bacteria using, A. Kamaladevi, A. Ganguli, and K. Balamurugan, “, D. H. Kim, R. Feinbaum, G. Alloing et al., “A conserved p38 MAP kinase pathway in, H. Pan and T. Finkel, “Key proteins and pathways that regulate lifespan,”, K. Neuhaus, M. C. Lamparter, B. Zölch et al., “Probiotic, M. Zhou, J. Zhu, H. Yu et al., “Investigation into in vitro and in vivo models using intestinal epithelial IPEC-J2 cells and, Y. L. Lu, “Screening and identification of lactic acid bacteria with acid and bile tolerance,”, F. Sabir, Y. Beyatli, C. Cokmus, and D. Onal-Darilmaz, “Assessment of potential probiotic properties of, M. Zhou, H. Yu, X. Yin, P. M. Sabour, W. Chen, and J. Gong, “, D. Uccelletti, E. Zanni, L. Marcellini, C. Palleschi, D. Barra, and M. L. Mangoni, “Anti-, T. I. Moy, A. R. Ball, Z. Anklesaria, G. Casadei, K. Lewis, and F. M. Ausubel, “Identification of novel antimicrobials using a live-animal infection model,”, C. Portal-Celhay, E. R. Bradley, and M. J. Blaser, “Control of intestinal bacterial proliferation in regulation of lifespan in, J. Zhu, X. Yin, H. Yu, L. Zhao, P. Sabour, and J. Gong, “Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic, K. J. Livak and T. D. Schmittgen, “Analysis of relative gene expression data using real-time quantitative PCR and the, G. Renaud, U. Stenzel, and J. Kelso, “leeHom: adaptor trimming and merging for Illumina sequencing reads,”, A. M. Bolger, M. Lohse, and B. Usadel, “Trimmomatic: a flexible trimmer for Illumina sequence data,”, X. Wang, Y. Chen, C. Gong, X. Yao, C. Jiang, and Q. Yang, “Molecular identification of four novel cytochrome P450 genes related to the development of resistance of, X. L. Li, C. Wang, D. F. Yu, L. R. Ding, W. Y. Zhu, and S. Q.